Human musclin Specification Purpose: This kit is used to determine the musclin content in human serum, plasma and related fluid samples. Principle of the experiment: This kit uses a double-antibody sandwich method to determine the level of human musclin in the specimen. A solid-phase antibody is prepared by coating a microtiter plate with a purified human musclin antibody, and musclin is sequentially added to the micropores of the coated monoclonal antibody, and then combined with HRP-labeled musclin antibody. An antibody-antigen-enzyme-labeled antibody complex is formed and after thorough washing, a substrate TMB is developed. TMB is converted to blue under the catalysis of the HRP enzyme and converted to the final yellow color by the action of acid. The depth of color is positively correlated with the musclin in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human musclin in the sample was calculated using a standard curve. Kit Composition: Kit Composition 48-well Configuration 96-well Configuration Storage Instructions 1 Part 1 Partial Closure 2 sheets (48) 2 sheets (96) Sealed bag 1 1 Enzyme Plate 1 x 48 1 x 96 2 -8°C Storage Standards: 900pg/ml 0.5ml × 1 Bottle 0.5ml × 1 Bottle 2-8°C Storage Standard Diluent 1.5ml × 1 Bottle 1.5ml × 1 Bottle 2-8°C Enzyme Label Reagent 3ml × × 1 bottle of 6 ml × 1 bottle 2-8 °C storage sample dilution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 °C preservation reagent A solution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 °C preservation Reagent B Liquid 3 ml × 1 Bottle 6 ml × 1 Bottle 2-8°C Storage Stop Solution 3ml × 1 Bottle 6ml × 1 Bottle 2-8°C Save Concentrate Wash Solution (20ml × 20x) × 1 Bottle (20ml × × 30 times)×1 bottle 2-8°C