Human Parathyroid Hormone ELISA Kit (PTH) Experimental Operation Description Principle The kit uses a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). To the coated microwells pre-coated with human parathyroid hormone (PTH) capture antigen, specimens, standards, and HRP-labeled detection antibodies were sequentially added, incubated and thoroughly washed. Using the substrate TMB to develop color, TMB is converted to blue under the catalysis of peroxidase and converted to the final yellow color by the action of an acid. The color depth is positively correlated with human parathyroid hormone (PTH) in the sample. The absorbance (OD value) was measured at 450 nm using a microplate reader to calculate the sample concentration.
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Human parathyroid hormone ELISA test kit (PTH) experimental operation instructions
1. Remove the required slats from the foil pouch after equilibrating for 20 min at room temperature. The remaining slats are sealed back to 4 °C with a ziplock bag.
2. Set standard hole, sample hole and blank hole, blank hole is added; standard product hole is added with different concentration of standard product 50μL;
3. The sample hole to be tested is first added with 10 μL of the sample to be tested, and then 40 μL of the sample diluent is added;
4. Then add 50 μL of horseradish peroxidase (HRP)-labeled detection antibody to the standard well and sample well (without blank). Seal the well with a sealing membrane, 37 ° C water bath or incubator temperature Breed for 60min.
5. Discard the liquid, pat dry on the absorbent paper, fill each well with the washing solution, let stand for 1 min, remove the washing solution, pat dry on the absorbent paper, and repeat the washing 5 times (can also be washed with a washing machine).
6. Add 50 μL of substrate A and B to all wells and incubate for 15 min at 37 ° C in the dark.
7. Add 50 μL of stop solution to all wells and measure the OD value of each well at a wavelength of 450 nm within 15 min.
Human parathyroid hormone ELISA test kit (PTH) experimental operation instructions Beijing Jiehui Biotechnology Co., Ltd. free test ELISA kit within a week results 010-57269780
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Our company provides free agency service (saving time for you), Chinese and English dual-page manual, you are welcome to call us (010-57269780 (13366469818) online QQ)
Human parathyroid hormone ELISA test kit (PTH) experimental operation instructions
1. Remove the required slats from the foil pouch after equilibrating for 20 min at room temperature. The remaining slats are sealed back to 4 °C with a ziplock bag.
2. Set standard hole, sample hole and blank hole, blank hole is added; standard product hole is added with different concentration of standard product 50μL;
3. The sample hole to be tested is first added with 10 μL of the sample to be tested, and then 40 μL of the sample diluent is added;
4. Then add 50 μL of horseradish peroxidase (HRP)-labeled detection antibody to the standard well and sample well (without blank). Seal the well with a sealing membrane, 37 ° C water bath or incubator temperature Breed for 60min.
5. Discard the liquid, pat dry on the absorbent paper, fill each well with the washing solution, let stand for 1 min, remove the washing solution, pat dry on the absorbent paper, and repeat the washing 5 times (can also be washed with a washing machine).
6. Add 50 μL of substrate A and B to all wells and incubate for 15 min at 37 ° C in the dark.
7. Add 50 μL of stop solution to all wells and measure the OD value of each well at a wavelength of 450 nm within 15 min.
Human parathyroid hormone ELISA test kit (PTH) experimental operation instructions Beijing Jiehui Biotechnology Co., Ltd. free test ELISA kit within a week results 010-57269780
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