M. pneumoniae Antibody (MP Ab) Technical Instructions:
1. Before use, remove the kit from the refrigerator and allow it to reach room temperature for 15–30 minutes. If the enzyme-labeled strip is not immediately used after opening, store it in a sealed bag to maintain its integrity.
2. The concentrated washing solution may form crystals when stored. When preparing the working solution, gently heat it in a water bath until fully dissolved. This will not affect the test results.
3. Use a dedicated pipette for each step and regularly check the accuracy to minimize errors. Try to complete sample loading within 5 minutes. For large sample volumes, it's recommended to use an automatic pipette for better efficiency and consistency.
4. Always prepare a standard curve during each test, and it’s best to run duplicate wells. If the sample OD value exceeds that of the first standard well, dilute the sample with a known factor (n times) before testing. When calculating, multiply the final result by the dilution factor (×n×5).
5. The sealing film is for single-use only to prevent cross-contamination between different tests.
6. Keep the substrate solution away from direct light to ensure stability and accuracy.
7. Follow the manual instructions strictly. The final results should be based on the microplate reader readings, not visual judgment.
8. Treat all samples, washes, and waste materials as biohazardous substances. Proper disposal procedures must be followed.
9. Do not mix reagents from different batches, as this may lead to inconsistent or unreliable results.
10. In case of any conflict between language versions, the English manual takes precedence.
These guidelines are essential for ensuring accurate and reliable M. pneumoniae antibody testing. Following them carefully helps maintain the quality of the assay and reduces the risk of errors. It's also important to maintain a clean and organized workspace to avoid contamination and improve overall laboratory efficiency. Regular training and calibration of equipment can further enhance the precision of the results. Always record your procedures and findings for future reference and troubleshooting.
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