Guangrui Bio, a leading domestic manufacturer of high-quality ELISA kits, offers an advanced Human Skeletal Muscle Receptor Tyrosine Kinase (MUSK) ELISA Kit. This kit is designed for precise and reliable detection of MUSK levels in various biological samples. To ensure accurate results, proper sample preparation and handling are essential.
Sample Preparation Guidelines:
1. **Serum**: Allow blood to clot at room temperature for 10–20 minutes, then centrifuge at 2000–3000 rpm for approximately 20 minutes. Carefully collect the supernatant. If precipitation occurs during storage, re-centrifuge before use.
2. **Plasma**: Use EDTA or sodium citrate as anticoagulant. Mix gently for 10–20 minutes, then centrifuge at 2000–3000 rpm for about 20 minutes. Collect the supernatant carefully and re-centrifuge if needed.
3. **Urine**: Collect using a sterile tube, then centrifuge at 2000–3000 rpm for 20 minutes. Carefully remove the supernatant and re-centrifuge if precipitate forms.
4. **Cell Culture Supernatant**: For secreted components, collect in a sterile tube and centrifuge at 2000–3000 rpm for 20 minutes. For intracellular components, dilute cell suspension with PBS (pH 7.2–7.4), lyse by repeated freezing and thawing, then centrifuge again to collect the supernatant.
5. **Tissue Specimens**: Weigh the tissue, add PBS (pH 7.4), homogenize, and centrifuge at 2000–3000 rpm for 20 minutes. Collect the supernatant and store the remaining portion at -20°C for future use.
6. **General Handling**: Process samples immediately after collection. If not tested right away, store at -20°C and avoid repeated freeze-thaw cycles. Note that samples containing NaN3 should not be used, as it may inhibit horseradish peroxidase (HRP).
ELISA Procedure Steps:
1. **Standard Dilution and Loading**: Prepare a serial dilution of standards on the ELISA plate. Add 100 μL of standard solution to the first two wells, then perform a 1:2 dilution across the remaining wells. Final concentrations will be 9, 6, 3, 1.5, and 0.75 μg/L.
2. **Sample Addition**: Set up blank control wells (no sample or enzyme reagent). Add 40 μL of sample diluent followed by 10 μL of the sample to each test well. Mix gently without touching the walls.
3. **Incubation**: Seal the plate and incubate at 37°C for 30 minutes.
4. **Washing**: Dilute the washing buffer according to instructions. Wash the plate 5 times, ensuring thorough removal of unbound substances.
5. **Enzyme Conjugate Addition**: Add 50 μL of enzyme-labeled reagent to all wells except the blank ones.
6. **Second Incubation**: Repeat the 37°C incubation for 30 minutes.
7. **Color Development**: Add 50 μL of color reagent A and B, mix gently, and incubate at 37°C for 15 minutes in the dark.
8. **Stop Reaction**: Add 50 μL of stop solution to each well to halt the reaction. The color will change from blue to yellow.
9. **Measurement**: Read the absorbance at 450 nm within 15 minutes of adding the stop solution. Ensure the microplate reader is properly zeroed before starting.
This comprehensive protocol ensures accurate and reproducible results when using the MUSK ELISA Kit from Guangrui Bio. Always follow the manufacturer's instructions and maintain strict quality control during the process.
Wings Furniture specializes in dining room furniture, and not only do we design each piece with care, but we also put a great deal of effort and passion into the manufacturing process. Wings Furniture's products are the best in terms of style and artistry on the outside, as well as quality and durability on the inside.
When it comes to dining tables, Wings Furniture offers a wide range of styles and sizes to meet the needs of different restaurants. From the simple and modern square Dining Table, to the vintage and elegant round dining table, to the majestic long dining table, each style has been meticulously crafted by the designers to bring the best dining experience to the users. At the same time, the materials used for the tables are carefully selected to ensure that they are both aesthetically pleasing and practical.
For chairs, Wings Furniture also spares no effort in providing a wide range of choices. From the luxurious texture of leather chairs, to the warmth and comfort of fabric chairs, to the natural simplicity of wooden chairs, every fabric and material has been carefully selected to bring users a richer dining experience. In addition, the dining chairs have been designed with ergonomics in mind to ensure that users remain comfortable even when dining for long periods of time.
For large wholesalers and retailers, Wings Furniture is an indispensable partner. Not only do they offer a wide range of products, but they are also very supportive in terms of price and service. Whether you want to buy in bulk to reduce costs or customize special styles to meet customer needs, you'll find the right products and solutions at Wings Furniture.
iron frame sofa chair,fabric dining room chair,Classic Casual Dining Chairs,Rebound Swivel Chair,swivel chair
Wings Meuble , https://www.wingsfurniture.com