Guangrui Bio, a domestic high-quality ELISA kit manufacturer, offers the Human Skeletal Muscle Receptor Tyrosine Kinase (MUSK) ELISA Kit, known for its excellent performance and reliability. Proper sample preparation is crucial for accurate results. Below are detailed guidelines for processing various specimen types:
1. **Serum**: Allow blood to clot at room temperature for 10–20 minutes, then centrifuge at 2000–3000 rpm for about 20 minutes. Carefully collect the supernatant. If precipitation occurs during storage, re-centrifuge before use.
2. **Plasma**: Use EDTA or sodium citrate as anticoagulants. Mix thoroughly after collection, then centrifuge at 2000–3000 rpm for 20 minutes. Collect the supernatant carefully, and re-centrifuge if needed.
3. **Urine**: Collect in a sterile tube and centrifuge at 2000–3000 rpm for 20 minutes. Carefully remove the supernatant, and re-centrifuge if precipitates form during storage.
4. **Cell Culture Supernatant**: For secreted components, collect in a sterile tube and centrifuge. For intracellular components, lyse cells by repeated freeze-thaw cycles after diluting with PBS (pH 7.2–7.4), then centrifuge again.
5. **Tissue Specimens**: Weigh the tissue, add PBS (pH 7.4), homogenize, and centrifuge. Store the supernatant at 2–8°C or freeze for later use. Avoid repeated freezing and thawing.
6. **General Sample Handling**: Process samples immediately after collection. If not tested right away, store at -20°C. Avoid multiple freeze-thaw cycles. Do not use samples containing NaN3, as it may inhibit HRP activity.
**Procedure Steps**:
1. **Standard Dilution and Loading**: Prepare a standard curve with 10 wells. Start with 100 μL of standard in the first two wells, then serially dilute using 50 μL of diluent. Continue this process to create concentrations of 9, 6, 3, 1.5, and 0.75 μg/L.
2. **Sample Addition**: Add 40 μL of sample diluent to each test well, followed by 10 μL of the sample (final dilution: 5x). Avoid touching the well walls and mix gently.
3. **Incubation**: Seal the plate and incubate at 37°C for 30 minutes.
4. **Washing**: Prepare a 30× (or 20× for 48T) diluted washing solution. Wash the plate 5 times, ensuring all liquid is removed and the plate is dried between each step.
5. **Enzyme Conjugate Addition**: Add 50 μL of enzyme-labeled reagent to each well except blank wells.
6. **Second Incubation**: Repeat the 37°C incubation for 30 minutes.
7. **Final Washing**: Perform the same washing steps as before.
8. **Color Development**: Add 50 μL of color developer A and B to each well. Mix gently and incubate at 37°C for 15 minutes in the dark.
9. **Stop Reaction**: Add 50 μL of stop solution to each well to terminate the reaction. The color will change from blue to yellow.
10. **Measurement**: Read the OD values at 450 nm within 15 minutes of adding the stop solution. Ensure the blank well is used for zero adjustment.
By following these detailed procedures, you can ensure accurate and reproducible results when using the MUSK ELISA Kit from Guangrui Bio. Always refer to the kit’s manual for specific instructions and safety information.
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In terms of color matching, we've gone the extra mile, offering everything from fresh and light macaron shades to calm and neutral tones to vibrant colors, each of which has been carefully selected and matched to add a splash of color or create a warm and harmonious atmosphere in your home.
More importantly, Wings Furniture insists on both high quality and cost-effectiveness. We use high-quality materials, combined with exquisite production technology, to ensure that each piece of sofa and chair has excellent durability and comfort. At the same time, we continue to optimize the cost control, and strive to bring more benefits to the majority of consumers, so that you can enjoy a high quality of life at the same time, but also feel the value for money shopping experience.
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