Common problems of high performance liquid chromatography

Many people doing chromatographic separation experiments have encountered such a situation that the mobile phase in the solvent bottle was sucked dry by accident and the mobile phase in the solvent bottle was inadvertently replenished. The HPLC system stopped working. Will this damage the column? Has the pump drained all mobile phase from the column? Can the column be used? In this article we provide you with satisfactory answers.

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