Gastrin (GAS), rat elisa gastrin (GAS), rat elisa Purpose: This kit is used to determine the content of gastrin (GAS) in rat serum, plasma and related fluid samples. Experimental principle: This kit uses the double antibody sandwich method to determine the level of gastrin (GAS) in the specimen. The microporous plate was coated with purified rat gastrin (GAS) antibody to prepare a solid phase antibody, and gastrin (GAS) was sequentially added to the microcapsules of the coated mAb, followed by HRP-labeled gastrin ( The GAS) antibody binds to form an antibody-antigen-enzyme-labeled antibody complex, which is thoroughly washed and then added to the substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with gastrin (GAS) in the sample. The absorbance (OD value) was measured at 450 nm using a microplate reader, and the rat gastrin (GAS) concentration in the sample was calculated from a standard curve. Kit composition: Kit composition 48-well configuration 96-well configuration Storage instructions 1 copy 1 part sealing film 2 pieces (48) 2 pieces (96) sealed bag 1 piece of enzyme-labeled plate 1×48 1×96 2 -8 ° C preservation standard: 72ng / L 0.5ml × 1 bottle 0.5ml × 1 bottle 2-8 ° C preservation standard dilution 1.5ml × 1 bottle 1.5ml × 1 bottle 2-8 ° C preservation enzyme standard reagent 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation sample dilution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation developer A solution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C preservation Reagent B Liquid 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation solution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation concentrated washing solution (20ml × 20 times) × 1 bottle (20ml × 30 times) × 1 bottle 2-8 ° C preservation
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