Studies have shown that bone marrow contains a variety of stem cells, which are also called bone marrow stem cells (BMSC). A large number of studies have been carried out by animal scholars on animal-derived BMSCs, and it has been confirmed that they can be differentiated into a variety of mesodermal-derived cells such as chondrocytes, osteoblasts and fat cells in vitro and in vivo. BMSC is currently the focus of tissue engineering seed cell research. As a tissue engineering seed cell, it has the following advantages: the material handling operation is relatively simple, and the damage to the body is small; after the bone marrow is extracted, the body can repair and replenish the corresponding cell components, so that the material can be repeatedly taken; the culture system is mature and convenient for popularization and application; in vitro induction technology Basically mature, easy to achieve the expected results. Therefore, to a certain extent, it can be said to be an ideal seed cell. Through minimally invasive materials, a small amount of seed cells are obtained, which are extensively expanded in vitro and then used for the construction of new tissues, thereby achieving a perfect surgical treatment effect of non-invasive or minimally invasive healing. At present, the research on BMSC has been deepened at home and abroad. A large number of literatures have reported its successful induction to chondrocytes, osteoblasts, and fat cells, and applied to tissue defect repair, and even initially applied in clinical practice. There are many related studies on the induction of differentiation of BMSC into chondrocytes by cartilage. Currently, the most inducible factors are: TGF-β (transforming growth factor-β, TGF-β) 1, 2, 3, bone morphogenetic protein (bone) Morphogenetic protein, BMP) 2, 6, 9, 13 , insulin-like growth factor-1 (IGF-1), cartilage-derived morphogenetic protein (CDMP) 1, 2 and Dexamethasone and the like. TGF-β mainly acts on the early stage of induction. It is generally believed that activation of the Smad pathway by its receptor activates the transcription of cartilage-specific genes, mainly by up-regulating the expression and synthesis of type II collagen. BMP2, 6 and so on can significantly enhance the expression and synthesis of aggrecan. IGF-l can significantly improve the potency of the above two types of factors, enhance their induction, and maintain the cartilage phenotype and function of the induced cells. CDMP is a type of inducing factor in the early stage of cartilage differentiation. It plays an important role in embryonic limb formation and cartilage development, and can promote the differentiation of undifferentiated mesenchymal cells into chondrocytes. Dexamethasone is a multi-efficiency inducing factor that promotes differentiation into chondrocytes, osteoblasts, and adipocytes. In addition, some factors that contribute to the differentiation of chondrocytes also have different degrees of induction, such as low oxygen concentration, low serum concentration or serum-free culture and high-density aggregation culture. However, most of the current studies are limited to cartilage-induced differentiation in vitro, and most of the in vivo experiments are performed in the joint, which may be related to the induction of ectopic (such as subcutaneous) cartilage in vivo. Studies have shown that after TGF-p1 and dexamethasone induction, BMSC can express chondrocyte-specific extracellular matrix components in large quantities, but after mixing with pluronic, it can not be injected into nude mice or pigs. The formation of mature cartilage tissue, but the formation of vascularized fibrous tissue; but injected into the joint environment, can form mature cartilage tissue, and even repair the full-thickness defect of articular cartilage. This indicates that the intra-articular microenvironment facilitates the differentiation of BMSCs into chondrocytes. In conclusion, although the mechanism by which BMSCs differentiate into chondrocytes is not fully understood, researchers have mastered a mature method for inducing differentiation into chondrocytes, and therefore, the possibility of widespread use in the future is greatly increased. 2. Osteogenic-induced BMSC can not only differentiate into bone, cartilage and other cells in vitro, but also maintain its osteogenic potential after long-term passage in vitro. Among the multi-directional differentiation potential of BMSC, its osteogenic capacity is particularly evident. When in vitro culture, p-phosphoglycerol, dexamethasone, and vitamin D: bone-inducing components are added to the culture medium, and BMSC gradually exhibits the phenotype of osteoblasts: the cells are fusiform and spontaneously form calcium salts. Deposition, formation of characteristic calcium nodules, macroscopic observation shows that a number of nodular structures are formed at the bottom of the culture dish. A variety of histological staining methods can demonstrate the presence of calcium nodules: alizarin red staining (ARSstain) forms red nodules, and vonKossa stains form black nodules. BMSC showed a significant increase in alkaline phosphatase 7 days after osteogenic induction. Various osteogenic specific proteins, such as osteocalcin (OCN), osteopontin (OPN), and bone sialoprotein (BSP), have also begun to be expressed. 3. BMSC repairs articular cartilage defects cartilage defects is a major problem in the field of trauma treatment. Articular cartilage defects caused by traumatic, tumor or cartilage degenerative diseases have no effective repair methods in the clinic, which will eventually lead to loss of joint function and even disability, causing serious physical and mental pain to patients. Although artificial joint replacement is currently very popular, its limited service life and prosthesis loosening and toxicity in the body have not been properly solved, especially for young patients. Therefore, the search for a biological resurfacing method in order to fundamentally solve this problem has become an urgent need in the clinic. For more than half a century, people have studied cartilage transplantation, perichondrium transplantation, periosteal transplantation and other repair methods, but most of them form pseudo-repair with fibrocartilage tissue, and with long-term degradation, can not meet the clinical requirements. . The success of in vitro chondrocyte culture has led to attempts to repair articular cartilage defects directly with chondrocytes. The chondrocyte suspension was injected into the defect of the articular cartilage. The results showed that the defect was fibrous tissue repair, and only a small number of neonatal chondrocyte nodules were seen under the microscope. BMSC has both cartilage and osteogenic potential, and theoretically it should be the best seed cell for repairing joint defects of articular cartilage and bone. A large-area full-thickness articular surface defect was made in the weight-bearing part of the joint, and then the autologous BMSC was extensively expanded in vitro and induced into cartilage, and then combined with the absorbable scaffold material polyglycolic acid to repair the joint defect. The results show that the defect has not only been well repaired, but its repair quality even surpassed the repair of autologous chondrocytes as seed cells, which showed cartilage defects as tissue engineered cartilage tissue repair, while deep bone defects were tissue engineering. Bone tissue repair. Therefore, BMSC can not only form cartilage and bone tissue in vitro, but also repair tissue defects in situ in vivo. 4. The initial application of BMSC in various clinical trauma and brain surgery is a common clinical disease. When the defect area is larger than 1/10 of the skull area, the defect cannot be repaired by surrounding bone tissue regeneration. At present, commonly used treatment methods such as autologous bone transplantation, allogeneic bone transplantation and various material repairs have different degrees of defects and lead to various complications. BMSC is used as a seed cell, which is combined with degradable materials and then transplanted into the body. With the gradual degradation of the material, the cells secrete the extracellular matrix and finally form a physiologically functional bone tissue, which is expected to become a new treatment mode for bone defect repair. . The use of natural absorbable materials (such as partially decalcified bone) as a scaffold, the use of in vitro osteogenic induced autologous hBMSC as seed cells, tissue engineering technology to repair human skull defects has been successfully carried out in China. Through three-dimensional CT and multiple detection techniques, the formation process of tissue engineered bone in the human body and the structure and biochemical composition of tissue engineered bone can be detected. The results of three-dimensional CT showed that the bone defect area was filled. Three to six months after operation, the three-dimensional CT showed that the human cell material complex formed a high-density shadow, suggesting that the tissue engineered bone formed and repaired the bone defect area. From 6 to 48 months after operation, the tissue engineered bone formed by three-dimensional CT showed stable existence, and no obvious bone resorption phenomenon was observed. CT scan showed the fusion of tissue engineered bone and adjacent bone. The results of histological examination showed that the periphery of the formed tissue-engineered bone was a normal trabecular-like structure, and the cartilage lacuna was seen, indicating that this is a typical cartilage internalization process. Immunohistochemical examination revealed that the new tissue also expresses bone tissue-specific proteins such as osteopontin and osteonectin. The application of tissue engineered bone in the repair of skull defects has shown its obvious advantages: the isolation of hBMSC from a small amount of bone marrow, the repair of large bone defects after large-scale expansion in vitro, and the change of bone source in the donor site in the current treatment of bone defects. The problem is to avoid further damage to the autologous bone donor area; after 2 years of follow-up, the formed tissue engineering bone is stable and the physiological function is normal. Compared with the traditional autologous bone graft treatment, tissue engineering technology can repair the defect without restriction of the defect area, and does not cause tissue defect and dysfunction in the donor area. Therefore, it can achieve the purpose of non-invasive repair of large-area bone defect and is easily accepted by patients.
Aerial Working Truck, Aerial work truck, Sky lift truck, Overhead working truck transport refers to the staff and equipment to the site to be used and air operations of special vehicles.
use
Aerial Working Truck Crank arm type overhead working truck can overhanging operation, across some obstacles or in a lift can be carried out more work; Platform capacity big, for two or more people work at the same time and can carry a certain device; Lift platform mobility, convenient transfer site; Beautiful shape, suitable for indoor and outdoor operation and storage. Applicable to the station, wharf, shopping malls, sports venues, residential property, factories workshop and so on a wide range of aerial work.
Folding boom overhead working truck trailer type, mobile convenience, folding boom compact structure, adopts the new type of high quality steel, high strength, light weight, direct access to the alternating current (ac) or use dc power start itself, construction speed is quick, table can be increased and horizontal extension, also can rotate, the obstacles to reach the working position, is the ideal aerial work equipment.
In both assignments in the bucket and rotary seat is equipped with control devices, remote control of engine start/stop, high speed/low speed, adopting electro-hydraulic proportional valve control arm movements, good stability, working arm can be right and left 360 째 continuous rotation, tank level automatic linkage for homework, main pump failure emergency pump down homework groove can be manipulated, and night lighting, lifting heavy objects. Car leg alone separate, adjustable, can realize the vehicle on uneven road leveling. Can the vehicle mobile and convenient, suitable for narrow area, a side operations. Products are widely used in electricity, street lamp, municipal, gardens, communications, airport, built (revised) ship, transportation, advertising, photography, and other areas of the aerial work.
Multifunctional and multipurpose
Through the big arm front bracket, can be quickly installed lifting device or platform, realize the material function such as lifting, lifting, and manned aerial work, at the same time to extension assignments device and a variety of work device quickly switch provides the interface.
3 d rotation lift
Design three-dimensional rotating lift device, already can keep by lifting material profile automatically, and can realize the lifting materials in space any height, any location and any direction adjustment request, sensitive speed control precision, micro performance is good, can meet the large underground structure in high altitude and ventilation pipe installation requirements.
Aerial Working Truck classification
According to the form of its lifting mechanism, generally can be divided into the telescopic arm type (ZhiBeiShi), folding arm type (crank arm type), vertical lift and hybrid four basic forms, etc.
Aerial Working Truck,High-altitude Operation Truck,Overhead Working Truck,Jmc Aerial Working Truck,Bucket Work Truck
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